Modern flow cytometry: a practical approach.

Flow Cytometry: A Novel Approach for Indirect Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies

Flow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency

Background Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. FCM provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. During spermiogenesis histones are replaced by prota...

Clinical flow cytometry, a hypothesis-driven discipline of modern cytomics.

Recently, two major books have been published that summarize the historical aspects and recent achievements of practical flow cytometry (1,2). Both emphasize the role played by this newly developed technical discipline in the development of scientific (1) and diagnostic platforms during late 20th-century medicine (2). Indeed, the gray box called a flow cytometer is the result of a multidiscipli...

Practical issues in high-speed cell sorting.

Modern flow cytometric cell sorters are all capable of so-called "high-speed sorting." However, there is confusion about exactly how fast a "high-speed" cell sorter can sort cells. There are many considerations in achieving the fastest sorting speed, as well as the highest quality sort results--cell recovery, purity, and functionality. This requires the same considerations required for "slow-sp...

Practical Flow Cytometry

The title of this book, Practical Flow Cytometry, may be somewhat misleading; a significant portion of the information presented is not essential to most investigators who utilize flow cytometry. The author's broad expertise in electronics, optics, histochemistry, and biology provides an excellent basis for discussion of the principles of flow cytometry and cell sorting; however, successful app...